Enhanced Methane Production from Pretreatment of Waste Activated Sludge by Economically Feasible Biocatalysts

Crude hydrolytic extracellular enzymes (CHEEs) generated by a mixed culture of microorganisms during fermentation have high potential as economically feasible biocatalysts for the hydrolysis complex organic wastes. This study investigates feasibility CHEEs substitutes commercial based on series anaerobic batch tests CH4 production fed pretreated waste activated sludge (WAS). The results showed that cellulase presented highest yield 99.1 mL·CH4/g·COD WAS among samples with single enzymes, 34% higher than control sample. A diversity used in pretreatment led to from WAS. sample mixture four (amylase + protease lipase, APCL) 216.0 prepared resulted 211.9 WAS, which is comparable performance APCL. using different APCL concentrations was 0.34 g·APCL/g·COD